They will secure a spot at the knockout stages of this competition with a positive result, and can take the top spot with a victory.
With that in mind, this is our predicted XI for the Super Eagles' clash with Guinea-Bissau.
Nigeria predicted XI vs Guinea-Bissau
After his solid performance in Nigeria's victory over Ivory Coast, Stanley Nwabali will likely be Nigeria's first-choice goalkeeper moving forward. He will be expected to put up another good performance against Guinea-Bissau.
Being the Super Eagles' captain, William Troost-Ekong‘s place in the starting XI was never in doubt. The PAOK defender scored the winning goal when Nigeria played against Ivory Coast last week.
However, Troost-Ekong faces a late fitness test, and is questionable for Nigeria's final group game.
On that note, Kasimpasa's Kenneth Omeruo will likely take his place in Peseiro's starting XI.
Calvin Bassey was also phenomenal in Nigeria's second game. With six tackles, three clearances and an interception, the Fulham player should start on Monday.
The Super Eagles lined up in a 3-4-3 formation against Ivory Coast, and that three-man defence seemed to work for Jose Peseiro. Semi Ajayi was part of a defensive line that managed to prevent a free-scoring Ivory Coast from getting on the scoresheet.
Zaidu Sanusi got the nod to start the Super Eagles' first two matches, and quite frankly, he hasn't really impressed so far. The FC Porto player will have a chance to redeem himself against Guinea-Bissau.
Ola Aina started both of Nigeria's games in this competition. With two clearances, four tackles and an interception, the Nottingham Forest player was one of the best players on the field against Ivory Coast.
To be fair, Alex Iwobi's performance against the Ivorians wasn't
as great as his performance in Nigeria's AFCON opener.
However, the former Arsenal player has been a regular for the Super Eagles, scoring ten times in almost 70 appearances. Iwobi was also part of Nigeria's squad that won bronze at AFCON 2019.
Frank Onyeka looked really solid in Nigeria's previous two games, completing 88 percent of his passes on both occasions. The Brentford player has earned his place in the starting XI.
It's worth mentioning that Moses Simon was replaced by Samuel
Chukwueze in Nigeria's starting XI vs Ivory Coast.
However, the Nantes player was subbed on just after the 70-minute mark, after a lacklustre performance by Chukwueze.
Simon is expected to regain his place in the starting lineup, he scored the only goal in Nigeria's most recent encounter with Guinea-Bissau.
Another player who looked really good in Nigeria's first two ganes, Ademola Lookman has played his part, with five key passes and a couple of big chances created.The Atalanta player also provided an assist in Nigeria's 1-1 draw with Nzalang Nacional of Equatorial Guinea.
Will Victor Osimhen start in Nigeria's clash with Guinea-Bissau? That's not a serious question. The Napoli forward has been the key to the Super Eagles' success in this tournament.
Not only did he score Nigeria's only goal in their Nations Cup opener, the Super Eagles' other goal came from a penalty, with was awarded because of a foul on Osimhen.